Single-Ended HPLC Technology
Single-Ended Column Design
Traditional HPLC columns often present technical hurdles, including clogging, leakage, and complex installation processes. These issues lead to inefficient workflows and potential sample loss, underscoring the need for innovation in column design.
Clogging at the tube–column junction
Sorting through many available high-pressure fittings
Selecting the proper tube length and ID
Leakage around the high-pressure solvent connections
Installation problems with tubing to achieve minimum dead volume
Not enough space on a short column for a full-size label
Introducing the format
Introducing the SIELC Single-Ended HPLC Column
The design completely eliminates the need for external fittings and tubing while minimizing dead volume. Even if a leak occurs, the mobile phase remains contained within the system, preventing external leakage.
These columns are used in the Cromite™ analyzer and are compatible with standard HPLC systems.
All SIELC and traditional phases are available in the new format:
Reverse and Normal Phases, Ion Exchange, HILIC, Chiral, Ion Exclusion, and Size Exclusion.
Technology principle
How It Works
At the core of the SIELC Single-Ended HPLC Column lies a proprietary patented coaxial design, engineered for superior performance and reliability.
This unique architecture ensures near-zero dead volume and precise flow alignment, maintaining stable mobile phase movement across all flow rates and pressures.
Near-Zero Dead Volume
Ensures optimal chromatographic separation efficiency.
Precise Flow Alignment
Guarantees stable mobile phase movement, even at high pressures.
Simplified Installation
Reduces connection errors and streamlines setup processes.
Seamless Operation: Install Once, Forget About Fittings
As the mobile phase enters through the MP inlet, it flows through an internal axial capillary aligned with the column, passes through the packing bed, and exits through a bypass channel connected to the outlet. This design eliminates dead volume and prevents external leakage.
