HPLC Method for Analysis of Vanillin on Primesep SB
Method Summary
| System | Cromite™ HPLC System |
| Column | Primesep SB |
| Column Size | 3.2 x 100 mm, 5 µm, 100 A |
| Part Number | SB-32.115.0510 |
| Mobile Phase | MeCN – 20% |
| Buffer | AmFm pH 3.0 – 30 mM |
| Flow Rate | 0.5 ml/min |
| Injection Volume | 1 ml |
| Detection | UV 275 nm |
Application Details
Vanillin is an organic compound with the molecular formula C8H8O3. It is what gives vanilla beans their scent and flavor. It is often used in food preparation as flavoring or in fragrances for it’s scent. Due to the scarcity of the natural compound, artificial versions have been developed.
Vanillin can be retained and analyzed using the Primesep SB stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a ammonium formate buffer. Detection is performed using UV.
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