HPLC Method for Analysis of Simazine on Primesep 100
Method Summary
| System | Cromite™ HPLC System |
| Column | Primesep 100 |
| Column Size | 3.2 x 100 mm, 5 µm, 100 A |
| Part Number | 100-32.115.0510 |
| Mobile Phase | MeCN – 50% |
| Buffer | H2SO4 – 0.1% |
| Flow Rate | 0.5 ml/min |
| Injection Volume | 2 ml |
| Detection | UV 275 nm |
Application Details
Simazine is an herbicide with the molecular formula C7H12ClN5. Typically, it is used to control various annual weeds in established crops. It is a pre-emergence herbicide, meaning that it has to be applied prior to the weeds emerging, as it is ineffective against grown plants.
Simazine can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a sulfuric acid buffer. Detection is performed using UV.
Need help with this method?
Contact our team for method development, column selection, or application support.