HPLC Method for Analysis of Amoxicillin on Primesep 100
Method Summary
| System | Cromite™ HPLC System |
| Column | Primesep 100 |
| Column Size | 3.2 x 100 mm, 5 µm, 100 A |
| Part Number | 100-32.115.0510 |
| Mobile Phase | MeCN – 50% |
| Buffer | H2SO4 – 0.1% |
| Flow Rate | 0.5 ml/min |
| Injection Volume | 2 ml |
| Detection | UV 275 nm |
Application Details
Amoxicillin is a prescription antibiotic with the molecular formula C16H19N3O5S. It is typically used to treat bacterial infections and is taken orally.
Amoxicillin can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a sulfuric acid buffer. Detection is performed using UV.
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