HPLC Method for Analysis of 6-Benzylaminopurine on Newcrom R1
Method Summary
| System | Cromite™ HPLC System |
| Column | Newcrom R1 |
| Column Size | 3.2 x 100 mm, 5 µm, 100 A |
| Part Number | NR1-32.115.0510 |
| Mobile Phase | MeCN – 50% |
| Buffer | H2SO4 – 0.2% |
| Flow Rate | 0.5 ml/min |
| Injection Volume | 1 ml |
| Detection | UV 275 nm |
Application Details
6-Benzylaminopurine is a plant growth regulation compound with the molecular formula C12H11N5. It’s presence in asparagus, for example, leads to deeper color, increased firmness, and a decrease in fibrous hardness. In general, it stimulates cell division and differentiation.
6-Benzylaminopurine can be retained and analyzed using the Newcrom R1 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a ammonium formate buffer. Detection is performed using UV.
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