HPLC Method for Analysis of 4-Amino-2-nitrophenol on Primesep 100
Method Summary
| System | Cromite™ HPLC System |
| Column | Primesep 100 |
| Column Size | 3.2 x 100 mm, 5 µm, 100 A |
| Part Number | 100-32.115.0510 |
| Mobile Phase | MeCN – 50% |
| Buffer | H2SO4 – 0.1% |
| Flow Rate | 0.7 ml/min |
| Injection Volume | 2 ml |
| Detection | UV 275 nm |
Application Details
4-Amino-2-nitrophenol is a hydrocarbon with the molecular formula C6H6N2O3. It is a red-purple powder that is typically used in as an oxidation base in hair dye. California considers this compound a carcinogen.
4-Amino-2-nitrophenol can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a sulfuric acid buffer. Detection is performed using UV.
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